Table 21-1. Development of tall fescue expressed sequence tags (ESTs) from complementary DNA (cDNA) libraries constructed from different plant tissues and growing conditions.
cDNA libraries |
Number of ESTs |
Library description |
Stem |
5035 |
Shoots from field grown KY-31 (E-)† plants at E1-E3 and R1-R3 growth stages |
Root |
5689 |
Roots from 25 KY-31 (E-) plants collected after 3-10 wk of growth |
Leaf |
4939 |
All leaves of primary tiller of KY-31 (E-) plants grown in greenhouse |
Young seedling |
4784 |
3-7 d old seedlings of KY-31 (E-) plants from germinator |
Floral meristem |
5014 |
Floral meristems (3-15 mm) of field grown KY-31 plants collected in spring |
Drought stress shoot |
5051 |
Whole shoots from drought stressed and restressed KY-31 (E-) plants |
Drought stress root |
4992 |
Roots from drought stressed and restressed KY-31 (E-) plants |
Heat stress shoot |
5119 |
4-wk-old KY-31 (E-) plants heat stressed (44°C) in growth chamber |
Field stress shoot |
1211 |
Shoots from two tillers of 3 KY-31 plants grown at 35.2-38.7°C |
Heat stress SSH |
2495 |
SSH¶ library constructed between heat tolerant and sensitive tall fescue accessions |
Total |
44,329 |
Young seedling, floral meristem, root, leaf, stem, and stress tolerant libraries |
† KY-31 (E-), Kentucky 31 endophyte free genotypes.
¶ SSH, suppression subtractive hybridization.
<--DNA Sequences |