Table 21-1. Development of tall fescue expressed sequence tags (ESTs) from complementary DNA (cDNA) libraries constructed from different plant tissues and growing conditions.

cDNA libraries

Number of ESTs

Library description

Stem

5035

Shoots from field grown KY-31 (E-)† plants at E1-E3 and R1-R3 growth stages

Root

5689

Roots from 25 KY-31 (E-) plants collected after 3-10 wk of growth

Leaf

4939

All leaves of primary tiller of KY-31 (E-) plants grown in greenhouse

Young seedling

4784

3-7 d old seedlings of KY-31 (E-) plants from germinator

Floral meristem

5014

Floral meristems (3-15 mm) of field grown KY-31 plants collected in spring

Drought stress shoot

5051

Whole shoots from drought stressed and restressed KY-31 (E-) plants

Drought stress root

4992

Roots from drought stressed and restressed KY-31 (E-) plants
in greenhouse

Heat stress shoot

5119

4-wk-old KY-31 (E-) plants heat stressed (44°C) in growth chamber

Field stress shoot

1211

Shoots from two tillers of 3 KY-31 plants grown at 35.2-38.7°C

Heat stress SSH

2495

SSH¶ library constructed between heat tolerant and sensitive tall fescue accessions

Total

44,329

Young seedling, floral meristem, root, leaf, stem, and stress tolerant libraries

† KY-31 (E-), Kentucky 31 endophyte free genotypes.

¶ SSH, suppression subtractive hybridization.

<--DNA Sequences

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Tall Fescue Monograph